Thp1 assay
WebNov 13, 2024 · THP-1 cells are growing in complete RPMI as ATCC suggested, and we usally use THP- 1 cells less than P10. For the migration assay, I re-suspend THP-1 cells in serum … WebMay 11, 2015 · Understanding how immune cells such as macrophages interact with cancer cells is of increasing interest, as cancer treatments move towards combining both …
Thp1 assay
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WebTHP-1. TIB-202 ™. THP-1 is a monocyte isolated from peripheral blood from an acute monocytic leukemia patient. This cell line can be used in immune system disorder research, immunology research, and toxicology research. Note: These are suspension cells and floating cells are viable. WebApr 16, 2024 · Scale bars represent 200 μm for wound healing assay images and 50 μm for transwell assay images. e The CM of THP1-derived TAMs significantly promoted the self-renewal of PCSCs, while CCL5 NA ...
WebTHP1 is a monocytic cell line and monocytes usually have low to ... have been reported between THP-1 and PBMCs including migration in response to chemoattractants in a … WebTHP1-Null cells are derived from THP-1 human monocytic cells, that represent the most commonly used model cell line for the study of inflammasome activation as they express …
WebMultiTox-Fluor Multiplex Cytotoxicity assays were performed using four increasing concentrations of DUBs-IN2 inhibitor in THP-1 macrophages as indicated on the figures. Cells were treated with either vehicle control or DUBs-IN2 for either a 2- or 24-hour period, after which the assay buffers were added and incubated for 30 minutes before … WebIn addition, The transwell assay was conducted by adding macrophages to the upper chamber. After 24 h, The number of macrophages in the CCL5-treated group increased significantly (Figure 5A and B), indicating that CCL5 …
Web1 day ago · Given the critical role of ADAM10 in S. aureus pathogenesis, we performed cell-based metalloprotease assays to identify the effective inhibitors of ADAM10 from a natural compound library established in our laboratory (Fig. 1 (a)). We initially screened 500 compounds to look for potential active candidates that inhibited ADAM10 activity by at …
WebDec 31, 2024 · For in vitro osteoclast differentiation assays, RAW264.7 cells were seeded at a density of 25,000 cells per well in 24‐well tissue culture ... NF‐κB reporter assay. THP1‐Dual cells were maintained in culture medium containing 10 µg/ml blasticidin and 100 µg/ml Zeocin to maintain selection pressure and passaged every three ... current american men tennis playersWebNov 1, 2014 · THP-1 cell line is a suitable in vitro cell model to study modulation of monocyte and macrophage functions.. The use of THP-1 cell line can help supporting the eventual in vivo proven bioactivities of food compounds and pharmacological activities of drugs.. THP-1 cell line can hint to potential responses that might occur in vivo, however, … current american league batting averagesWeb9 hours ago · ß-lactamase assays were performed in THP1 infected cells as previously described 41. Around 10 5 THP-1 cells were seeded in a 96-well plate and differentiated for 72 hours using 50 µg/ml phorbol ... current american heart association guidelinesWebJul 9, 2015 · The levels of (a) interleukin (IL)-2; (b) tumor necrosis factor (TNF)-α; (c) IL-10; and (d) IL-12 were measured in a THP1 cell line using a Bio-Plex Human Cytokine Th1/Th2 Assay. Particulate matter was collected in 2013 from 8 to 10 March, on 19 and 20 March, from 8 to 15 April, and from 30 April to 6 May. current american flag designerWebTHP-1. TIB-202 ™. THP-1 is a monocyte isolated from peripheral blood from an acute monocytic leukemia patient. This cell line can be used in immune system disorder … current american prisoners in russiaWebInvivoGen offers several engineered THP-1 cell lines: THP1 NF-κB cells were specifically designed for monitoring the NF-κB signal transduction pathway in a physiologically … THP1-Lucia™ ISG cells were derived from the human monocytic cell line THP-1, … THP1-defCASP1 cells, together with the control cell line THP1-Null, allow to … THP1-NLRC4 cells are derived from the THP-1 human monocytic cell line. They … Details. THP1-Null2 cells are suited for the study of inflammasome activation. The … THP1-Difluo™ hLC3 reporter cells are designed to monitor the autophagic flux … Examining the endogenous expression of CXCL10 (IP10) THP1-Blue™ KI-IP10 … Unlike their parental cells, THP1-Dual™, which weakly express TLR9, THP1 … IRF7 knockout dual reporter monocytes. THP1-Dual™ KO-IRF7 cells were … current american red cross cpr guidelinesWebOct 27, 2024 · Kinase assay. WT THP1 cells were lysed in lysis buffer (1% (V/V) Nonidet P-40, 50 mM MOPS, pH 7.5, 5 mM MgCl 2, 0.5 mM MnCl 2, 100 mM NaCl, 0.4 mM Pefabloc SC, 0.1% (V/V) 2-mercaptoethanol) and ... current american men\u0027s tennis players