Phospho-jnk molecular weight

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August undefined, 2024

WebNov 1, 2024 · In our study, high glucose, instead of upregulating phospho-JNK and total JNK, significantly enhanced the phosphorylation levels of p38 in podocytes and diabetic mice and was effectively suppressed by ZGW. Therefore, ZGW is likely to inhibit podocyte apoptosis by regulation of the p38 MAPK pathway. WebAug 29, 2024 · p-JNK Antibody (G-7) is a mouse monoclonal IgG 1 κ, cited in 1,396 publications, provided at 200 µg/ml Anti-p-JNK Antibody (G-7) is recommended for detection of Thr 183 and Tyr 185 phosphorylated JNK1, JNK2 and JNK3 of mouse, rat and human origin by WB, IP, IF, IHC (P) and ELISA

phospho-JNK1/2 (pThr183/pTyr185) antibody Western J4644

Web36-8800 is specific for the ~42k - 44k ERK/MAPK phosphorylated at Thr202 and Tyr204. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. The immunolabeling is completely eliminated by lambda-phosphatase. Target Information WebThis MILAN8R antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded human tissue using low pH antigen retrieval and by immunocytochemistry of MeOH-fixed cells and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the … cumberland adventures

Jnk Sigma-Aldrich

WebAlso known as MAPK8 and SAPK (stress-activated protein kinase), these proteins are involved in an array of cellular processes and cell signal transmission. They respond to stress stimuli and aid in T cell differentiation and apoptosis. JNKs have 10 isoforms derived from 3 genes: JNK1, JNK2, and JNK3. WebRayBio® Phospho- JNK (T183/Y185) ELISA kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human, mouse and rat cell lysates. By determining phosphorylated JNK protein in your experimental model system, you can verify pathway activation in your cell lysates. Web7 rows · 80024-1-RR targets Phospho-JNK (Tyr185) in WB, ELISA applications and shows … cumberland advisors youtube

Anti-JNK1 (phospho T183) antibody (ab47337) Abcam

Anti-c-Jun (phospho S73) antibody (ab30620) Abcam

WebAug 23, 2012 · Detects a band of approximately 40, 60 kDa (predicted molecular weight: 48 kDa). Notes; WB 1/500 - 1/1000. Detects a band of approximately 40, 60 kDa (predicted molecular weight: 48 kDa). Target. ... WebRabbit polyclonal to JNK1 (phospho T183) Suitable for: WB, ELISA, ICC/IF, IHC-P Reacts with: Mouse, Rat, Human Isotype: IgG You may also be interested in Primary Anti-JNK1 antibody [EPR140 (2)] (ab110724) Secondary Goat Anti-Rabbit IgG H&L (HRP) (ab205718) Primary Anti-Albumin antibody [EPR20245] - Low endotoxin, Azide free (ab222923) east pensacola heights clubhouse pensacola flWebGuided by the molecular weight (MW 624), the extract was suspended in water (500 mL) and successively partitioned by n-Hexane and CH 2 Cl 2 for three times. ... phospho-ERK (p-ERK) (C) and phospho-JNK (p-JNK) (D) bands by densitometry. The values are expressed as mean ± SD from three independent experiments. east pensacola heights center

"Web63.44 KB Proteins Akt Western Blot Most recent answer 8th Aug, 2024 Viviane Augusto Australian National University When working with HepG2 cells I only see 40 kDa band using p-AKT antibody. Anyone... " - Phospho-jnk molecular weight

Phospho-jnk molecular weight

NF-kB p65 Protein Overview: Sequence, Structure, Function and …

WebAnti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] Description Rabbit monoclonal [EP1597Y] to JNK1 (phospho Y185) + JNK2 … WebUse at an assay dependent concentration. Detects a band of approximately 50 kDa (predicted molecular weight: 48 kDa).

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WebPhospho-JNK1/JNK2 (Thr183, Tyr185) Antibody (44-682G) in ICC/IF Immunofluorescent analysis of JNK1/2 (pT183/pT185) was done on 70% confluent log phase A549 cells treated with Anisomycin (25 µg/mL for 30 min). WebPhospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb detects endogenous levels of p46 and p54 SAPK/JNK when phosphorylated at Thr183 and Tyr185. It will also react with SAPK/JNK singly phosphorylated at Tyr185. This antibody may cross-react with phosphorylated p44/42 or p38 MAP kinases. Species Reactivity:

WebThe lower molecular weight band is phospho-JunD (Ser100). Confocal immunofluorescent images of C6 cells, untreated (left) or anisomycin treated (right), labeled with Phospho-c … WebOct 29, 2007 · The present study was undertaken to investigate whether isoforms of c-Jun N-terminal kinase (JNK 46 kDa and 54 kDa), one component of the mitogen-activated protein kinase (MAPK) family, might show region-related differential activation patterns in both naïve and pain-experiencing rats.

WebMar 10, 2024 · p-ERK Antibody (E-4) is an IgG2a κ mouse monoclonal p-ERK antibody (also designated p-ERK antibody or p44/42 MAPK antibody) suitable for the detection of ERK 1 phosphorylated at Tyr 204 and correspondingly phosphorylated ERK 2 of mouse, rat and human origin by WB, IP, IF, IHC (P) and ELISA. p-ERK Antibody (E-4) is available as the … WebDec 26, 2024 · The control value of phospho-JNK was higher than other factors, and the level of phospho-JNK in the AHC 1 mg/mL treatment group (0.77 ± 0.04) was 1.2-fold of control group (0.63 ± 0.05). This indicates that the expression of phospho-JNK protein was higher than those of other factors in MCF-7 cells and JNK was more activated by …

WebApr 7, 2024 · Particularly, Anisomycin is a JNK activator, which increases phospho-JNK. Obviously, Anisomycin is a bacterial antibiotic. In the second place, Anisomycin with 60 mg/kg for 4-week by iv significantly decreases mouse body weight in a dose-related manner. Additionally, Anisomycin with 15 mg/kg slightly and transiently decreases the mouse body …

WebMonoclonal Anti-MAP Kinase, Activated/monophosphorylated (Phosphothreonine ERK-1&2) antibody produced in mouse. ~2 mg/mL, clone ERK-PT115, purified immunoglobulin, … cumberland advisors sarasotaWebJNK, by phosphorylation, modifies the activity of numerous proteins that reside at the mitochondria or act in the nucleus. Downstream molecules that are activated by JNK … cumberland additive incWebNov 5, 2024 · The membranes were blocked with 5% skim milk in Tris-buffered saline containing 0.1% Tween 20, incubated with primary antibodies against P38, phospho-P38, ERK1/2, phospho-ERK1/2, JNK, phospho-JNK (1:1000, Cell Signaling Technology, Danvers, MA, USA) at 4 °C for overnight, and again incubated with secondary antibodies for 2 h at … east pensacola heights clubhouseWebMolecular Weight 46, 54 Cell Signaling Technology 3 Trask Lane Danvers, Massachusetts 01923 United States Phone: 877-616-CELL Phone: 978-867-2388 Fax: 866-432-6112 Fax: 978-867-2488 Company Profile Website: http://www.cellsignal.com/ Website: Technical Support Citations (542) east penn tow trucks mercerWebKey features and details Rabbit polyclonal to p38 (phospho T180 + Y182) Suitable for: IHC-P, ICC/IF, WB Reacts with: Rat, Human Isotype: IgG Get better batch-to-batch reproducibility … cumberland advocate newspaperWeb(1) Background: The c-Jun-NH2-terminal protein kinase (JNK) is a mitogen-activated protein kinase involved in regulating physiological processes in the central nervous system. However, the dual genetic deletion of Mkk4 and Mkk7 (upstream activators of JNK) in adult mice is not reported. The aim of this study was to induce the genetic deletion of … cumberland advisory groupWebFeb 19, 2024 · Little is known about the effects on hyaluronan (HA) metabolism of UVA radiation. This study demonstrates that the secretion of HA by human dermal fibroblasts (HDFs) is downregulated by UVA, accompanied by the down- and upregulation of mRNA and protein levels of the HA-synthesizing enzyme (HAS2) and the HA-degrading protein, … east penn self storage fleetwood