Webb7 juli 2011 · The open reading frame (ORF) encoding mutant TEAS was introduced into the Gateway expression vector pHis9-GW, and expressed under the same induction … WebbPlasmids. Our lab has constructed a series of plasmids that enable tagging of genes at the N- or C- terminus with fluorescent proteins or epitope tags for protein analysis, described …

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WebbThe open reading frame (ORF) encoding mutant TEAS was introduced into the Gateway expression vector pHis9-GW, and expressed under the same induction conditions as the wild-type enzyme. The mutant TEAS, however, formed inclusion bodies that required the following solubilization treatment with N -lauroylsarcosine. 47 WebbpHISi载体价格3000元，pHISi载体质粒图谱(Vector map)，载体序列(Sequence)见下文。 birthday puns funny

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Webb21 okt. 2009 · A unique product in the reaction of the recombinant enzyme from E. coli containing pHIS9-At5g41040 was detected (Fig. 3 A), which has a UV spectrum similar to ferulate, but its positive atmospheric pressure chemical ionization (APCI)-ion mass spectrum gives an molecular ion [M+H] + at an m/z of 449, indicating that the ferulate is … WebbIntroduction. The chloroplasts of vascular plants replicate by binary fission, similar to their cyanobacterial relatives, but utilize a machinery that is a composite of cyanobacterial and host-derived components (Osteryoung and Vierling, 1995; Kuroiwa et al., 1998; Miyagishima et al., 2001; Osteryoung, 2001; Gao et al., 2003; Osteryoung and Nunnari, … Webb27 juni 2006 · IGS1 and EGS1 cDNAs were also recombined with the Gateway-compatible, T7 expression vector pHIS9 (P. O’Maille, personal communication) through a pENTR (Invitrogen) intermediate to produce a protein with an N-terminal His 9 tag. Heterologous Protein Expression in E. coli and Purification. birthday puns one liners