Phenomenex clarity otx 100 mg 96-well plate
WebTo test proof of concept or for low sample volumes, Clarity OTX is available as a starter kit, which in- cludes either a 96-well plate or 50 solid phase extraction cartridges and all the buffers (lysis-loading, equilibration, wash, and elution) required for the extraction protocol. U.S. Patent No. 7, 119, 145 henene 379 Clarity® WebCytiva's Whatman UNIFILTER 2 mL, 96 well microplates are used for large sample or reagent volumes, as in biomolecule purification by SPE and organic synthesis in combinatorial …
Phenomenex clarity otx 100 mg 96-well plate
Did you know?
WebApr 20, 2024 · Cells were plated at 100 000 cells/well in a 24-well plate in 1 ml medium and oligonucleotides transfected 1.5 h after plating using Lipofectamine™ 2000. ... Aliquots were immediately quenched with 350 μl OTX Clarity Lysis buffer (Phenomenex #AL0-8579) and frozen at –20°C. Clean-up was conducted all at once using the Clarity® OTX ... WebSPE isolation cartridge (Clarity OTX 100mg/ 3mL tube; Phenomenex) is first “wetted” with methanol then equilibrated with Clarity OTX equilibration buffer (10 mM Phosphate pH 5.5) prior to sample loading. After sample loading the cartridge is rinsed twice with equilibration buffer followed by rinses with Clarity OTX
WebKS0-8494 Clarity OTX Starter Kit- Tubes Includes: 100 mg/3 mL cartridges (x50) Lysis-loading buffer (60mL) Equilibration buffer (250mL) Wash buffer (350mL) Elution buffer … WebMar 5, 2024 · Briefly, the analyte of interest is denatured and hybridized in solution with the capture probe and is subsequently attached to a plate via avidin-biotin association. Next, the detection probe is...
WebApr 14, 2024 · DPSCs were seeded on coverslips in 24-well plates (1.5 × 10 4 cells per well) and cultured at 37 °C overnight. The culture media were replaced with serum-free media … WebStep Solvent 100 mg 96-Well Plates 3 mL Cartridges Condition MeOH 1 mL 1 mL Equilibrate 50 mM Ammonium Acetate (pH 5.5) 1 mL 1 mL Load Pre-treated sample Up to 0.5mL 0.4 …
WebClarity Oligo-RP™. Clarity Oligo-RP columns have been specifically designed for the reversed phase purification of oligonucleotides with balanced hydrophobicity and polar …
WebJul 1, 2013 · A 96-well clarity OTX plate was installed on a SPE manifold, and a vacuum step of approximately 3 mmHg was applied. The plate was conditioned with 1 mL of methanol (HPLC grade), followed by 1 mL of equilibration solution (50 mM ammonium acetate, 2 mM sodium azide, pH 5.5). shoe cabinets for entryway solid woodWebThe Clarity OTX protocol is different from other SPE methods in that the sorbent not only binds the desired oligonucleotide analyte, but it also binds most other components in serum (proteins, lipids, oligos, etc.) before serially eluting them according to … raceline wheels 887WebConical V- and round-bottom for maximized sample delivery. Available in conical V- and round-bottom formats. Made of chemically inert polypropylene. Available in 350 μL, 1, and 2 mL volumes. Order Tab. Documents. raceline wheels 929mWebThe Clarity BioSolutions sample preparation portfolio is composed of unique solid phase extraction chemistries packed in 96-well plate and cartridge formats designed to … shoe cabinets argosWebWidest selection from a single source. choice of well volumes ranging from 80 μL to 10 mL, well densities from 24 to 384 wells with round or ′V′ bottom for maximum recovery. … shoe cabinets for entryway singaporeWeb2.2 固相萃取（Phenomenex Clarity OTX™ 96-well plate, 100 mg/well） 活化： 1mL 甲醇 平衡： 1mL平衡缓冲液 上样： 400 μL样品 淋洗: 1mL 淋洗缓冲液× 3次 洗脱: 2 × 500 μL 洗脱缓冲液 洗脱液经氮气吹干后，用100 μL复溶液溶解后离心上样。 （复溶液：10mM DIPEA+25mM HFIP in H 2 O:MeOH=9 ... raceline wheels 936 throttleWebClarity OTX lysis buffer and SPE cartridges were obtained from Phenomenex Inc. (CA, USA). Preparation of stock & working solutions Oligonucleotide solution was prepared in deionized water to give a final concentration of 2 mg/ml. IS stock solution concentration was 300 μg/ml. Working solutions of the oligonucleotides were prepared by spiking ... shoe cabinets for hallways ebay